Turkish Journal of Veterinary & Animal Sciences
DOI
-
Abstract
The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in E. coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. E. coli was isolated from 48% (48/100) of the samples. DNA was extracted from these isolates and was amplified by PCR, using a pair of primers derived from the eaeA (virulence) gene. In the agarose gel examination of PCR products, 48% (48/100) of the isolates were determined to have this virulence factor.
Keywords
Escherichia coli, PCR, chicken, eaeA gene
First Page
215
Last Page
218
Recommended Citation
KILIÇ, AYŞE; ERTAŞ, HASAN BASRİ; MUZ, ADİLE; ÖZBEY, GÖKBEN; and KALENDER, HAKAN (2007) "Detection of the eaeA Gene in Escherichia coli from Chickens by Polymerase Chain Reaction," Turkish Journal of Veterinary & Animal Sciences: Vol. 31: No. 4, Article 1. Available at: https://journals.tubitak.gov.tr/veterinary/vol31/iss4/1
