Turkish Journal of Veterinary & Animal Sciences
Comparison of Polymerase Chain Reaction and Conventional Methods for the Diagnosis of Listeria monocytogenes in Stuffed Mussels
The aim of this study was to evaluate the role of stuffed mussel as a source of Listeria monocytogenes. Polymerase chain reaction is a rapid procedure with both sensitivity and specificity for quick detection and identification of L. monocytogenes. A total of 50 mussel samples were investigated for L. monocytogenes. L. monocytogenes was not identified by the conventional method. However, PCR amplification products demonstrated that 5 out of 50 samples showed positive reactions with L. monocytogenes. The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.
Listeria monocytogenes, stuffed mussel, conventional methods, PCR
GÖKSOY, ERGÜN Ö.; KIRKAN, ŞÜKRÜ; and KAYA, OSMAN (2006) "Comparison of Polymerase Chain Reaction and Conventional Methods for the Diagnosis of Listeria monocytogenes in Stuffed Mussels," Turkish Journal of Veterinary & Animal Sciences: Vol. 30: No. 2, Article 13. Available at: https://journals.tubitak.gov.tr/veterinary/vol30/iss2/13