Turkish Journal of Veterinary & Animal Sciences
DOI
-
Abstract
The optimum UV duration times of eggs were examined in order to develop a simple and safe method for inducing androgenetic development in the Nile tilapia, Oreochromis niloticus. The yield of androgenetic haploid O. niloticus to the pigmentation stage was 18.53 ± 5.3% (relative to controls) with an optimal UV irradiation dose of 540 Jm^{-2} (at 150 µWcm^{-2 }) for 6 min. Most embryos developing after fertilisation with normal spermatozoa showed abnormal morphology and a haploid number of chromosomes (n = 22). The success of oocyte denucleation was also assessed by using the recessive "blond" skin pigmentation character.
Keywords
Androgenesis, chromosome manipulations, Nile tilapia, Oreochromis niloticus
First Page
401
Last Page
407
Recommended Citation
KARAYÜCEL, İSMİHAN and KARAYÜCEL, SEDAT (2003) "Optimisation of UV Treatment Duration to Induce Haploid Androgenesis in the Nile tilapia (Oreochromis niloticus L.)," Turkish Journal of Veterinary & Animal Sciences: Vol. 27: No. 2, Article 18. Available at: https://journals.tubitak.gov.tr/veterinary/vol27/iss2/18
