Turkish Journal of Veterinary & Animal Sciences
Abstract
The optimum UV duration times of eggs were examined in order to develop a simple and safe method for inducing androgenetic development in the Nile tilapia, Oreochromis niloticus. The yield of androgenetic haploid O. niloticus to the pigmentation stage was 18.53 ± 5.3% (relative to controls) with an optimal UV irradiation dose of 540 Jm^{-2} (at 150 µWcm^{-2 }) for 6 min. Most embryos developing after fertilisation with normal spermatozoa showed abnormal morphology and a haploid number of chromosomes (n = 22). The success of oocyte denucleation was also assessed by using the recessive "blond" skin pigmentation character.
DOI
-
Keywords
Androgenesis, chromosome manipulations, Nile tilapia, Oreochromis niloticus
First Page
401
Last Page
407
Recommended Citation
KARAYÜCEL, İ, & KARAYÜCEL, S (2003). Optimisation of UV Treatment Duration to Induce Haploid Androgenesis in the Nile tilapia (Oreochromis niloticus L.). Turkish Journal of Veterinary & Animal Sciences 27 (2): 401-407. https://doi.org/-