Turkish Journal of Veterinary & Animal Sciences
Abstract
In this study, a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method, and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be
DOI
-
Keywords
Testosterone, enzyme immunoassay
First Page
45
Last Page
50
Recommended Citation
GÜVEN, B, ÖZSAR, S, MARAŞLI, N, MARAŞLI, Ş, & ÖZCAN, A (2003). Development of an Enzyme Immunoassay for the Determination of Testosterone. Turkish Journal of Veterinary & Animal Sciences 27 (1): 45-50. https://doi.org/-
