Development of an Enzyme Immunoassay for the Determination of Testosterone

Authors

BÜLENT GÜVEN
SEMİN ÖZSAR
NALAN MARAŞLI
ŞABAN MARAŞLI
AYLA ÖZCAN

DOI

-

Abstract

In this study, a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method, and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be

Keywords

Testosterone, enzyme immunoassay

First Page

45

Last Page

50

Recommended Citation

GÜVEN, BÜLENT; ÖZSAR, SEMİN; MARAŞLI, NALAN; MARAŞLI, ŞABAN; and ÖZCAN, AYLA (2003) "Development of an Enzyme Immunoassay for the Determination of Testosterone," Turkish Journal of Veterinary & Animal Sciences: Vol. 27: No. 1, Article 7. Available at: https://journals.tubitak.gov.tr/veterinary/vol27/iss1/7