Detection and RFLP Analysis of Canine Parvovirus (CPV) DNA by Polymerase Chain Reaction (PCR) in a Dog

Authors

AYKUT ÖZKUL
İHSAN KELEŞ
TANER KARAOĞLU
MEHMET ÇABALAR
İBRAHİM BURGU

DOI

-

Abstract

In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.

Keywords

Canine parvovirus, diagnosis, PCR, RFLP

First Page

1201

Last Page

1203

Recommended Citation

ÖZKUL, AYKUT; KELEŞ, İHSAN; KARAOĞLU, TANER; ÇABALAR, MEHMET; and BURGU, İBRAHİM (2002) "Detection and RFLP Analysis of Canine Parvovirus (CPV) DNA by Polymerase Chain Reaction (PCR) in a Dog," Turkish Journal of Veterinary & Animal Sciences: Vol. 26: No. 5, Article 37. Available at: https://journals.tubitak.gov.tr/veterinary/vol26/iss5/37