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Turkish Journal of Veterinary & Animal Sciences

DOI

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Abstract

The purpose of this study was to compare the birth rates after transfers of microinjected hybrid mouse zygotes into hybrid CB6F1 (C57BL/6J X BALB/c) and outbred CD-1 foster mothers. One or two hours after microinjection of a \beta -actin-Gfp gene construct into the male pronucleus, hybrid mouse zygotes were transferred into CB6F1 and CD-1 foster mothers. In addition, non-microinjected hybrid zygotes were transferred into foster mothers of both strains. The overall data demonstrate that outbred CD-1 foster mothers are a better choice for transfer of microinjected hybrid mouse embryos, and there was a statistical difference between the transfers of microinjected zygotes into CB6F1 and CD-1 foster mothers (P

Keywords

Microinjection, pronuclear stage, embryo transfer, hybrid, outbred

First Page

329

Last Page

333

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