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Turkish Journal of Veterinary & Animal Sciences

DOI

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Abstract

Polysaccharide-degrading enzymes of the anaerobic rumen bacterium Ruminococcus flavefaciens strain 17 were exmined. This work demonstrated that R. flavefaciens strain 17 produces a complex array of enzymes active against cellulose, hemicellulose (xylan) and lignin. These enzymes are xylanase, avicellase, acid-swollen cellulase, carboxymethyl cellulase (CMCase), pNP-cellobiosidase (pNPC), pNP-glucopyranosidase (pNPG), pNP-xylopyranosidase (pNPX) and pNP-arabinofuranosidase (pNPA). Although R. flavefaciens is an anaerobic bacterium, its enzymes worked optimally under aerobic conditions and with dithiothreitol (DTT), except in the case of pNP-xylopyranosidase. pNP-xylopyranosidase exhibited a higher level of activity under anaerobic conditions with DTT. It was demonstrated that the cross-links between arabinose side chains, which esterified via ferulic acid and p-coumaric acid, were cleaved by R. flavefaciens esterase and a-arabinofuranosidase.

First Page

703

Last Page

709

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