Acid glycine extract (AGE) and whole cell (WC) antibodies wer e used in ELISA testing for the detection of Campylobacter fetus subsp. venerealis and subsp. fetus antibodies in the blood sera of 150 female cattle and 100 bulls which wer e one year old or older . It was observed that AGE antibody was mor e specific than WC antibody . A cr oss r eaction was detected between C. fetus subsp. venerealis and subsp. fetus. Thus, it was concluded that for Campylobacter ELISA could only be used as a monitoring test. In dir ect diagnosis of C. fetus with the Indir ect Flur escent Antibody T est and A vidin-Biotin Staining, 36% and 31.2% positivity wer e detected in 125 samples of vaginal mucus. 42% and 40% positivity wer e detected with the same test in 100 samples of pr eputial fluid. The differ ences of 5% and 2% between the two positivity values wer e not found to be statistically significant. The AB me thod is suggested for dir ect diagnosis.
Campylobacter fetus, ELISA, IF A, A vidin-Biotin.
AK, SEYYAL; TURAN, NURİ; AK, KEMAL; İLERİ, İ. KAMURAN; and ILGAZ, ATİLLA (2000) "Use of ELISA, IF A, and A vidin-Biotin Staining for the Diagnosis of Bovine Genital Campylobacteriosis," Turkish Journal of Veterinary & Animal Sciences: Vol. 24: No. 2, Article 3. Available at: https://journals.tubitak.gov.tr/veterinary/vol24/iss2/3