One hundred pneumonic lungs from calves were examined by an immunoper oxidase technique using avidin-biotin- per oxidase complex method in formalin-fixed, paraffin-embedded sections for Haemophilus somnus. In the histological examination of tissue sections, strong positive reactions against H. somnus were detected in 14% of the lungs. H. somnus antigens were observed at the surface and/or within the epithelial cells, macrophages, necrotic alveolar walls, alveolar -bronchial/br onchiolar exudates, fibrins, distended interlobular septa and perivascular areas. In addition, dense antigen accumulations were detected in the necrotic macrophages and neutrophils.
HAZIROĞLU, RIFKI; ERDEĞER, JALE; GÜLBAHAR, M. YAVUZ; KUL, OĞUZ; and YILDIRIM, MURAT (2000) "Localization of Haemophilus somnus Antigen by an Immunoper oxidase Technique in Pneumonic Bovine Lungs," Turkish Journal of Veterinary & Animal Sciences: Vol. 24: No. 2, Article 13. Available at: https://journals.tubitak.gov.tr/veterinary/vol24/iss2/13