Turkish Journal of Veterinary & Animal Sciences
Abstract
Some biochemical properties of purified and non-purified rumen tissue arginase were compared. Homogenization, heating, treatment with aceton, precipitation with ammonium sulfate, dialysis, several centrifugations, gel filtration on sephadex G-200 processes were utilized in the purification procedure of the enzyme. It was found that pre-incubation temperature (60 °C) of arginase and Km (4mM) to its substrate, L-arginine, did not change before and after purification. While pre-incubation period was 10 min and optimal pH 9.7 before purification, pre-incubation period was found to be 5 min and optimal pH 10 after purification. Purified enzyme achieved its highest activity at 2 mM MnCl_2 concentration, whereas non-purified enzyme gave the highest activity at 1 mM MnCl_2 concentration. Consequently, it was determined that Mn^{++} cations and preincubation were essential for the activation of the enzyme.
DOI
-
Keywords
Cattle Rumen Tissue, Arginase, Purification, Kinetic properties
First Page
597
Last Page
608
Recommended Citation
ERİŞİR, MİNE and OZAN, SEMA TEMİZER (1999) "Some Properties of Purified and Non-purified Rumen Tissue Arginase in Cattle," Turkish Journal of Veterinary & Animal Sciences: Vol. 23: No. 9, Article 25. Available at: https://journals.tubitak.gov.tr/veterinary/vol23/iss9/25
