Turkish Journal of Veterinary & Animal Sciences




The effects of a long term qualitative and quantitative protein deficiency and following feeding a complete, standard commercial diet for mice and rats on the peripheral white blood cells of circa four months old female and male Wistar rats were investigated. In doing so, the animals was given first for 60 days a diet consisting of 10 or 20% gelatine and thereafter, for refeeding for 14 days, a complete chow diet for rats and mice. The concentrations of leukocytes were determined directly in the hemocytometry by the method ad modumRandolph (17), as it is lightly modified (16), and the results tested merely with a three-way analysis of variance with repetitions of measures in factor time where the duration of the experiment, the gelatine content of the diet and the sex of animals have been examined as possible influencing factors. With feeding of gelatine as protein source in the diet, the number of the neutrophils increased (p < 0,001), whereas the number of mononuclear leukocytes and eosinophils felt dramatically (p < 0,01 and p < 0,0001, respectively). There were no significant alterations on the number of total leukocytes. The followed refeeding with a complete rat and mouse chow diet led to an increase in the number of total leukocytes (p < 0,0001), eosinophils (p < 0,0001), neutrophils (p < 0,001) and mono nuclear leukocytes (p < 0,01). Also the differential blood picture showed significant alterations during both the protein deficiency and standard commercial rodent diet feeding periods.


Qualitative and quantitative protein deficiency, amino acid deficiency, gelatine, tryptophan, peripheral blood cells, leukocytes, rats.

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