Turkish Journal of Veterinary & Animal Sciences
Abstract
Ovine immunoglobulin M was isolated from sheep serum using gel filtration and affinity chromatography. Polyclonal and monoclonal antibodies against this isotype were prepared. The specificity of the monoclonal antibody was investigated using SDS-Page, immunoblotting, an enzyme-linked immunosorbent assay (ELISA) and affinity chromatography. On immunoblotting, monoclonal anti-IgM reacted with a 70 kD protein which corresponded to ovine IgM.
DOI
-
Keywords
IgM, Isolation, monoclonal, antibody.
First Page
135
Last Page
140
Recommended Citation
YILMAZ, H, TANTAŞ, A, ILGAZ, A, & MORGAN, K. L (1999). Isolation and Preparation of Monoclonal Antibody to OvineImmunoglobulin M. Turkish Journal of Veterinary & Animal Sciences 23 (2): 135-140. https://doi.org/-
