Turkish Journal of Veterinary & Animal Sciences
DOI
-
Abstract
Ovine immunoglobulin M was isolated from sheep serum using gel filtration and affinity chromatography. Polyclonal and monoclonal antibodies against this isotype were prepared. The specificity of the monoclonal antibody was investigated using SDS-Page, immunoblotting, an enzyme-linked immunosorbent assay (ELISA) and affinity chromatography. On immunoblotting, monoclonal anti-IgM reacted with a 70 kD protein which corresponded to ovine IgM.
Keywords
IgM, Isolation, monoclonal, antibody.
First Page
135
Last Page
140
Recommended Citation
YILMAZ, HÜSEYİN; TANTAŞ, AYSAN; ILGAZ, ATİLLA; and MORGAN, KENTON L. (1999) "Isolation and Preparation of Monoclonal Antibody to OvineImmunoglobulin M," Turkish Journal of Veterinary & Animal Sciences: Vol. 23: No. 2, Article 6. Available at: https://journals.tubitak.gov.tr/veterinary/vol23/iss2/6
