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Turkish Journal of Veterinary & Animal Sciences

Separation of Retinol Acetate, Vitamin A Palmitate, a- and g- Tocopherol by Reverse-Phase High Performance Liquid Chromatography and Determination of These Vitamins in Van Lake Fish (Chalcalburnus tarichi)

DOI

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Abstract

The resolution properties of several commercial columns for vitamin A and E standards by reverse phase chromatography methods have been examined. Hypersil MOS column with methanol-water (96:4) and acetonitrile-water (90:10) mobile phase, Shimadzu-ODS column with methanol-water (96:4) mobile phase have acceptable resoluion in separation of vitamin A esters. Shimadzu-ODS column/96 % methanol and Shimadzu-C8/90% acetonitrile have the acceptable resolution ratio for vitamin E isomers. Methanol extracts of heart muscle and gill in Van Lake fish (Chalcalburnus tarichi) have been examined for vitamin A and E by high performance liquid chromatography. Avarage retinol acetate, vitamin A palmitate, alpha tocopherol, gamma tocopherol in heart muscle of Van Lake fish, have been found as 162±23 ng/g, 18±2.2 ng/g, 8.5±1.0 µg/g, 505.7±94 ng/g, respectively. The vitamin levels in gills have been determined as retinol acetate 231.5±38 ng/g, vitamin A palmitate 23.8±3.5 ng/g, alpha tocopherol 12.4±0.77 µg/g, gamma tocopherol 350.1±97 ng/g. A significant differences have been found among the retinol acetate-vitamin A patmitate and alpha-gamma tocopherol values in heart muscles and gills (P A negative correlation for retinol acetate between heart muscle and gill (r:-.86), vitamin A palmitate between heart muscle and gill (r:-.73) and between retinol acetate-alpla tocopherol in heart muscles (r:-.71) have been observed. A positiv correlation between retinol acetate and alpha tocopherol in gills (r:+.52) has been found. No correlation has seen between heart muscles and gills for gamma tocopherol values.

Keywords

Vitamin A, vitamin E, HPLC, Fish, Chalcalburnus tarichi

First Page

17

Last Page

22

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