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Turkish Journal of Veterinary & Animal Sciences

Detection of IgA Producing Cells in Sheep Intestine Using Immunoperoxidase Staining

Authors

Hüseyin YILMAZ

DOI

-

Abstract

Samples from the duodenum, jejunum and ileum of 6 sheep were taken and fixed in formol saline. Sections from these tissues were used in immunocytochemistry studies. Plasma cells producing IgA in the small intestine were determined with rabbit-anti ovine IgA using immunoperoxidase staining. Cells with brown staining in 10 randomly selected area under microscope were counted using image analyser. Mean cell count of 10 microscope area of duodenum, jejunum and ileum was 43, 53.3 and 74 respectively.

Keywords

Sheep, Immunoglobulin, Plasma cells, Immunoperoxidase

First Page

73

Last Page

77

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