•  
  •  
 

Turkish Journal of Medical Sciences

Abstract

Background/aim: To determine the effects of an A2A receptor agonist regadenoson, an A2A receptor antagonist istradefylline, and istradefylline nanosuspension on ovarian ischemia/reperfusion (I/R) injury in rats.

Materials and methods: A total of 80 female rats were divided into 10 groups: sham, ovarian I/R, blank nanosuspension + ovarian I/R, regadenoson (3 μg/kg) + ovarian I/R, regadenoson (30 μg/kg) + ovarian I/R, istradefylline (0.3 mg/kg) + ovarian I/R, istradefylline (3 mg/kg) + ovarian I/R, istradefylline-loaded nanosuspension (3 mg/kg) + ovarian I/R, istradefylline (3 mg/kg) + regadenoson (30 μg/kg) + ovarian I/R, and istradefylline-loaded nanosuspension (3 mg/kg) + regadenoson (30 μg/kg) + ovarian I/R. ELISA, chemiluminescence, and spectrophotometric analyses were performed on blood and ovarian tissue samples. Histopathological changes were analyzed using hematoxylin and eosin staining, and a scoring system was applied to assess ovarian tissue damage.

Results: Serum malondialdehyde levels were augmented in the I/R and blank nanosuspension + I/R groups. Although tissue superoxide dismutase levels were decreased with I/R, these levels were increased with regadenoson (3 μg/kg), istradefylline (0.3–3 mg/kg), and istradefylline nanosuspension (3 mg/kg). While there was augmentation in the serum and tissue 3-nitrotyrosine levels in the I/R group, a marked decrease was identified with regadenoson (30 μg/kg, p < 0.05). Native thiol levels were decreased in the I/R group, and this decrease was prevented by regadenoson. Serum disulfide levels were increased in the I/R group, and this increase was suppressed by regadenoson.

Conclusion: These data showed that adenosine A2A receptors may contribute to the ovarian I/R injury and regadenoson can produce protective effects.

Author ORCID Identifier

AZAD MAMMADOV: 0000-0002-2416-5745

ABDULLAH DEMİRYÜREK: 0000-0002-9994-8541

AHMET SARACALOĞLU: 0000-0003-2534-6051

CAHİT DEMİRKIRAN: 0000-0003-3240-1549

İSMAİL DEĞİM: 0000-0002-9329-4698

ÖMER ERONAT: 0000-0001-6768-9998

ŞENİZ DEMİRYÜREK: 0000-0003-4762-4745

DOI

10.55730/1300-0144.6170

Keywords

Adenosine receptor, ischemia/reperfusion, istradefylline, Ovary, regadenoson

First Page

364

Last Page

376

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Download

Share

COinS