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Turkish Journal of Medical Sciences

Abstract

Background/aim: Breast cancer represents the foremost cause of cancer-related mortality among women worldwide and is characterized by a markedly high metastatic potential, contributing substantially to its clinical severity and poor prognosis. This study aimed to evaluate the interrelationship among silent information regulator (SIRT1), Forkhead box O3a (FoxO3a), and microRNA-34a (miR- 34a), the latter of which promotes apoptosis by suppressing the proliferation, migration, and invasion of breast cancer cells. Specifically, we investigated the tumor microenvironment associated with metastatic progression of malignant primary tumors, the influence of nonmetastatic (benign) tumors, and the potential roles of these proteins in both primary tumor development and metastasis.

Materials and methods: In this study, metastatic 4TLM and nonmetastatic 67NR breast cancer cell lines were used. These cell lines were orthotopically injected into the mammary fat pads of 8–10-week-old female Bagg Albino laboratory-bred strain c (BALB/c) mice. Mice were sacrificed 28 days postinjection, and primary tumors, lungs, and liver tissues were collected for analysis. Expression levels of SIRT1, FoxO3a, and miR-34a were evaluated using immunohistochemistry, Western blotting, and RT-PCR.

Results: Expression levels of SIRT1 and FoxO3a were significantly higher in metastatic 4TLM tumors compared to the nonmetastatic 67NR group. Conversely, miR-34a expression was markedly higher in nonmetastatic tumors, whereas its level was reduced in metastatic tissues (p < 0.05). In metastatic tissues, SIRT1 expression remained elevated, whereas FoxO3a and miR-34a levels were significantly reduced (p < 0.05).

Conclusion: SIRT1 may act as either a tumor suppressor or a tumor promoter, depending on cellular context, signaling pathways, and its specific molecular targets across cancer types. The elevated expression of SIRT1 in 67NR primary tumors suggests a tumor-suppressive role in nonmetastatic settings. However, the increased SIRT1 expression observed in metastatic regions indicates its potential role as a tumor promoter and modulator of the tumor microenvironment, possibly through FoxO3a and miR-34a signaling, thereby enhancing cellular proliferation and invasion.

Author ORCID Identifier

BANU ŞAHİN: 0009-0004-4208-4545

İLKER BURAL: 0009-0007-1760-9751

HÜSEYİN ÖZCAN: 0009-0008-9659-1891

SENDEGÜL YILDIRIM: 0000-0002-3359-3899

GAMZE TANRIÖVER: 0000-0002-8002-5544

ŞÜKRAN YOLDAŞ: 0000-0002-9491-3075

DOI

10.55730/1300-0144.6166

Keywords

Breast cancer, FoxO3, metastasis, miR-34a, SIRT1

First Page

326

Last Page

332

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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