•  
  •  
 

Turkish Journal of Medical Sciences

Abstract

Background/aim: The immune system functions as a well-coordinated defense mechanism, protecting the host from both external pathogens and internal threats. Cancer cells often display surface antigens that the immune system can recognize as foreign, potentially triggering an immune response. However, many cancer cells evade detection by downregulating or completely losing these surface antigens. The immune system relies on the expression of surface antigens and human leukocyte antigens (HLA) to identify and target tumor cells. One key method by which tumor cells evade natural killer (NK) cells involves alterations in HLA antigens. Colorectal cancer (CRC) is known to cause various changes in the immune system, including the increased expression of HLA antigens on cell surfaces, reduced functionality of NK cells, and mechanisms for immune evasion. The aim of this study was to investigate the possible roles of innate immunity and associated HLA-G molecules in the development of CRC by examining tumor tissue samples.

Materials and methods: We evaluated soluble HLA-G (sHLA-G) levels via ELISA, investigated HLA-G expression loss in tumor samples through immunohistochemistry (IHC), and assessed killer cell immunoglobulin-like receptor (KIR) expression on NK cells in tumor tissues.

Results: No significant correlation was found between HLA-G and sHLA-G levels (p = 0.641). Among patient samples, 16.7% (6 of 36) were positive for HLA-G, with varying intensities, while no staining was observed in control samples. Compared to control samples, IHC staining revealed a significantly higher rate of KIR positivity in CRC tissue samples. One notable finding of our study was the variability in KIR staining intensity within the same tumor. We observed differences in KIR expression not only between tumors, but also within distinct areas of the same tumor. Additionally, a significant relationship was found between KIR expression and age.

Conclusion: In conclusion, this study highlights the increased expression of both HLA-G and KIR markers in CRC patients, suggesting their potential as prognostic and predictive markers. Our findings also suggest that HLA-G and KIR molecules could represent valuable therapeutic targets for future cancer immunotherapy strategies.

Author ORCID Identifier

EZGİ DİNÇER: 0009-0001-8980-0375

FATMA KAYA DAĞISTANLI: 0000-0002-4672-8721

KIVANÇ DERYA PEKER: 0000-0002-8887-3505

DAMLANUR SAKIZ: 0000-0002-2051-1049

FİGEN ABATAY SEL: 0000-0002-1155-1284

DEMET KIVANÇ: 0000-0002-2451-5709

HAYRİYE ŞENTÜRK ÇİFTÇİ: 0000-0001-5160-5227

ÇİĞDEM KEKİK ÇINAR: 0000-0003-2098-381X

ŞULE KARATAŞ: 0000-0002-8605-4178

FATMA SAVRAN OĞUZ: 0000-0002-6018-8936

DOI

10.55730/1300-0144.6073

Keywords

colorectal cancer, Human leukocyte antigen-G, killer-cell immunoglobulin-like receptor

First Page

1188

Last Page

1196

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Download

Share

COinS