Vitamin D3 alleviates intestinal injury in necrotizing enterocolitis and lipopolysaccharide-induced inflammatory response in dendritic cells in rats

Authors

BO KEFollow
CHAOYU LIFollow
SONG LIFollow
JINGJING YANFollow
LEKE SUNFollow

Author ORCID Identifier

BO KE: 0000-0002-7288-4971

CHAOYU LI: 0009-0000-1036-7293

SONG LI: 0009-0006-4650-8039

JINGJING YAN: 0009-0009-3835-326X

LEKE SUN: 0009-0005-6344-2265

DOI

10.55730/1300-0144.5895

Abstract

Background/aim: Necrotizing enterocolitis (NEC) is a serious condition that predominantly affects premature infants and involves an aberrant immune response and inflammatory cytokine release resulting in intestinal epithelial damage. The current study investigated the immunoregulatory effects of vitamin D3 on the maturation and activation of dendritic cells (DCs) and the antiinflammatory impact on the intestines in a neonatal rat model of NEC.Materials and methods: Inflammatory damage to intestinal tissue was assessed via morphological changes and apoptosis and DC expression of costimulatory molecules, inflammatory factors, and immunoregulatory factors by immunohistochemical staining, quantitative real-time PCR, and immunofluorescence. The fluorescein isothiocyanate-ovalbumin (FITC-OVA) uptake assay was used to analyze DC endocytosis.Results: Vitamin D3 administration attenuated intestinal damage and apoptosis, inhibiting CD86 and increasing CD80 expression. Lipopolysaccharide (LPS)-challenged DC2.4 cells in vitro showed upregulated CD86, tumor necrosis factor-α (TNF-α), interleukin-1β (IL- 1β), inducible nitric oxide synthase (iNOS), and indoleamine 2,3-dioxygenase 1 (IDO-1) expression, which were all reduced by vitamin D3, except for IDO-1. LPS inhibited CD80 expression, which was restored by vitamin D3 treatment, and endocytic capacity was improved. Vitamin D3 ameliorated intestinal damage in neonatal rats with NEC and exerted antiinflammatory and immunomodulatory effects on DCs.Conclusion: Vitamin D3 has potential as a supplementary treatment for NEC patients.

Keywords

dendritic cells, inflammatory response, Necrotizing enterocolitis, vitamin D3

First Page

1165

Last Page

1174

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

KE, BO; LI, CHAOYU; LI, SONG; YAN, JINGJING; and SUN, LEKE (2024) "Vitamin D3 alleviates intestinal injury in necrotizing enterocolitis and lipopolysaccharide-induced inflammatory response in dendritic cells in rats," Turkish Journal of Medical Sciences: Vol. 54: No. 5, Article 34. https://doi.org/10.55730/1300-0144.5895
Available at: https://journals.tubitak.gov.tr/medical/vol54/iss5/34