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Turkish Journal of Medical Sciences

Author ORCID Identifier

ZERİN ÖZAYDIN AKSUN 0000-0003-1154-4276

SEYDA ERDOĞAN 0000-0003-2759-9562

AYŞE KALKANCI 0000-0003-0961-7325

ELİF AYÇA ŞAHİN 0000-0002-2215-2756

TUĞBA ÇUHADAR 0000-0002-6760-340X

H. ÖZDEN ŞENER 0000-0001-6825-6983

DOI

10.55730/1300-0144.5825

Abstract

Background/aim: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease. Several studies shown that alterations of microbiota, increase the risk of neurodegenerative disorders. We aimed to reveal whether there is a difference in the gut microbiota of patients with ALS.Materials and methods: The participants are divided into three groups. Group 1 formed patients with ALS. Healthy family members living in the same house of the patients formed Group 2. Lastly, sex, age-matched healthy people were included in Group 3. Fecal samples were collected in 15 mL falcon tubes and stored at -80 °C. Genomic DNA isolation was performed on samples. Bacterial primers selected from the 16S rRNA region for the bacterial genome and ITS1 and ITS4 (Internal Transcribed Spacer) were used for the identification of DNA. Next generation sequence analysis (NGS), taxonomic analyzes were performed at the level of bacterial phylum, class, order, family, genus, and species. Alpha and beta diversity indexes were used. The Linear Discriminant Analysis (LDA) Effect Size method (LEfSe) was applied to identify a microbial taxon specific to ALS disease.Results: The relative abundances of Succinivibrionaceae and Lachnospiraceae families were significantly lower in patients. The dominant families of patients were Streptococcaceae and Ruminococcaceae, while the dominant families of healthy controls were Bacteroidaceae, Succinivibrionaceae. LEfSe analysis showed four families (Atopobiaceae, Actinomycetaceae, Erysipelatoclostridiaceae, Peptococcacceae) differed significantly between the patients and healthy controls (LDA values> 2.5, P <0.05).Conclusion: Comparison with family members living in the same house is the strength of this study. We found that there were changes in the microbiota of the patients, consistent with the literature. Studies that analyze the composition of the gut microbiota in the pre-disease period may be needed to understand whether dysbiosis is caused by the mechanisms inherent in the disease or whether it is dysbiosis that initiates the disease.

Keywords

ALS, Amyotrophic lateral sclerosis, gut microbiota, neurodegenerative diseases, pathogenesis of ALS

First Page

579

Last Page

587

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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