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Turkish Journal of Medical Sciences

DOI

10.55730/1300-0144.5761

Abstract

Chemical biological radiological nuclear threats are at an important point in the agenda of world health today, as theycan cause mass deaths. B. mallei attracts attention as a potential biological warfare agent due to its features such as multidrug resistance,a rapid transmission mechanism via aerosol, the absence of a complete treatment protocol for the infection it causes, and the absence ofan approved vaccine for protection against the bacteria. B. mallei suspect samples must be studied by experienced personnel in biosafetylevel III laboratories. B mallei is a difficult and troublesome pathogen to diagnose and many unknowns about B. mallei today. Therefore,the aim of the study was to determine the molecular differences and potential resistance genes of B mallei strains.Materials and methods: Determination of the molecular differences and potential resistance genes of B mallei strains with newbioinformatics approaches by comparatively examining the data of 29 B mallei strains, 10 of which were isolated from Türkiye, on thegenome list of the National Biotechnology Information Center (NCBI).Results: According to the genome annotations of the origins, the origin containing the highest number of CDS which is 5172 was foundas the 11th strain obtained in Türkiye in 1949. The origin with the highest number of pseudogenes was determined as 23,344 (China 7)origin. Two hundred and eighty-five pseudogenes found in this strain were obtained from a knee effusion in Myanmar. According tochromosome 2 data, B. mallei strain was determined as the most similar strain to ATCC 23344, line 11 with NCTC 10229 strain, andSAVP1 strain was determined as the least similar strain. When the antimicrobial resistance gene markers of the isolates included in thestudy were examined, amrA and amrB, qacG ade, Burkholderia pseudomallei Omp38 were found to be carrying.Conclusion: In terms of public health, it was thought that the data obtained as a result of our study about B mallei, which is defined asa biological weapon, is very valuable for creating treatment protocols to be applied to possible epidemics in the future. In addition, theavailable genetic epidemiological data of these strains belonging to a category that is dangerous to work with in a laboratory environmentwere reviewed.

Keywords

B. mallei, chemical biological radiological nuclear threats, glanders, whole genome sequence

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16

Last Page

25

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