Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species

Authors

IŞIN AKYAR
CENGİZ ÇAVUŞOĞLU
MELTEM AYAŞ
SÜHEYLA SÜRÜCÜOĞLU
ZEYNEP ARZU İLKİ
DENİZ ECE KAYA
YEŞİM BEŞLİ

DOI

10.3906/sag-1801-198

Abstract

Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis. Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study. Results: Identification by MBT ML4.0 was correctly performed in 100% of MTC and in 91% of NTM isolates. All of the MTC isolates were correctly differentiated from NTM isolates. Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.

Keywords

MALDI-TOF MS, identification, Mycobacterium, Mycobacterium tuberculosis complex, nontuberculous mycobacteria

First Page

1351

Last Page

1357

Recommended Citation

AKYAR, IŞIN; ÇAVUŞOĞLU, CENGİZ; AYAŞ, MELTEM; SÜRÜCÜOĞLU, SÜHEYLA; İLKİ, ZEYNEP ARZU; KAYA, DENİZ ECE; and BEŞLİ, YEŞİM (2018) "Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species," Turkish Journal of Medical Sciences: Vol. 48: No. 6, Article 37. https://doi.org/10.3906/sag-1801-198
Available at: https://journals.tubitak.gov.tr/medical/vol48/iss6/37