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Turkish Journal of Medical Sciences

DOI

10.3906/sag-1803-121

Abstract

Background/aim: ß-Lactamase-producing Escherichia coli strain JJ1886 is an epidemic clone with high virulence properties. Because this strain can survive in the bloodstream, we aimed here to understand how ß-lactam antibiotics and human serum affect the growth and gene expression of this bacterium. Materials and methods: We report the time-dependent growth effect of normal human serum and heat-inactivated serum, together with ß-lactam antibiotics (including cefotaxime, ceftazidime, and carbenicillin), for E. coli strain JJ1886. Relative gene expression of ß-lactamase-related genes (encoding the ß-lactamase regulator, CTX-M-15, and peptidoglycan glycosyltransferase) and serum survival- associated genes (encoding lipoprotein NlpI, murein lipoprotein, lipopolysaccharide core heptose (I) kinase, lipopolysaccharide biosynthesis protein, capsule synthesis protein, and phosphate transport system) were investigated by RT-qPCR. Results: Cells proliferated during the exponential growth phase when the bacterium was treated with human serum. However, cefotaxime and ceftazidime together with serum had a bactericidal effect at each of the tested time points. Downregulation was observed in gene-encoding lipoprotein NlpI as a result of treatment with carbenicillin. Conclusion: Serum plus cefotaxime or ceftazidime had bactericidal activities. When the bacterium was treated with human serum and ß-lactam antibiotics, there were no significant changes in relative gene expression, except for the nlpI gene.

Keywords

Escherichia coli JJ1886, ß-lactamase, serum, RT-qPCR, cefotaxime, ceftazidime, carbenicillin

First Page

1340

Last Page

1350

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