Ankaferd Blood Stopper with antibiofilm potential successfully inhibits the extracellularmatrix degradation enzymes and promotes wound healing of 3T3 fibroblasts in vitro

Authors

RUKİYE BORAN
TUBA BAYGAR
NURDAN SARAÇ
AYSEL UĞUR

DOI

10.3906/sag-1712-80

Abstract

Background/aim: The potential inhibitory effects of Ankaferd Blood Stopper (ABS) against biofilm formation of oral microorganisms and its capacity for collagenase, hyaluronidase, and elastase inhibitions that have important roles in wound healing have been investigated. Materials and methods: The wound healing potential was determined by its inhibition ability on collagenase, hyaluronidase, and elastase enzyme activities and was evaluated via scratch wound healing assay on murine 3T3 fibroblasts. The antibiofilm activity was tested against eight oral microorganisms using the crystal violet staining method. Results: At 10% ABS successfully inhibited the biofilm formation of the tested microorganisms. Enzyme inhibition analysis revealed that 3% ABS significantly inhibited all three enzymes related to wound healing. The scratch assay showed that wound closure was faster than that of the control for the 3% ABS/plate. Conclusion: The findings of the present study indicated that ABS has effective wound healing potential with its strong antibiofilm activity against oral cavity microorganisms.

Keywords

Ankaferd Blood Stopper, enzyme inhibition, antibiofilm, wound healing

First Page

627

Last Page

634

Recommended Citation

BORAN, RUKİYE; BAYGAR, TUBA; SARAÇ, NURDAN; and UĞUR, AYSEL (2018) "Ankaferd Blood Stopper with antibiofilm potential successfully inhibits the extracellularmatrix degradation enzymes and promotes wound healing of 3T3 fibroblasts in vitro," Turkish Journal of Medical Sciences: Vol. 48: No. 3, Article 26. https://doi.org/10.3906/sag-1712-80
Available at: https://journals.tubitak.gov.tr/medical/vol48/iss3/26