Effects of di erent drug treatments on the proliferation of human ovarian carcinoma cell line MDAH-2774

Authors

ŞULE AYLA
AYHAN BİLİR
AHMET ŞENOL ERTÜRKOĞLU
GAMZE TANRIVERDİ
BURAK CEM SONER
KENAN SOFUOĞLU
LAURA GHISOLFI
GÜLPERİ ÖKTEM

DOI

10.3906/sag-1507-193

Abstract

Background/aim: In this study, the e ects of resveratrol as a natural polyphenol compound, gemcitabine as an antimetabolite that has nucleoside structure analogous to deoxycytidine, and para-aminophenol-derived paracetamol were investigated with single and combined applications in monolayers of the MDAH-2774 human ovarian cancer cell line. Materials and methods: Drugs were evaluated in cell culture with respect to cell proliferation, cell cytotoxicity (trypan blue dye exclusion test), synthesis phase of cell cycle, and cell structure in 24, 48, 72, and 96 h. Result: Resveratrol and gemcitabine diminished both cell proliferation and cell cycle synthesis phase indication in monolayer cell cultures (P < 0.05). All combination groups showed similar e ects that were mainly more e ective in respect to single usage of resveratrol and gemcitabine in monolayer cell cultures. Conclusion: The effects of gemcitabine, resveratrol, and paracetamol were investigated in monolayers of the MDAH-2774 human ovarian cancer cell line and a decrease in cell number in cell cycle synthesis phase, prevention of cell proliferation, and destruction of cell structure were observed.

Keywords

Ovarian cancer, gemcitabine, paracetamol, resveratrol

First Page

441

Last Page

448

Recommended Citation

AYLA, ŞULE; BİLİR, AYHAN; ERTÜRKOĞLU, AHMET ŞENOL; TANRIVERDİ, GAMZE; SONER, BURAK CEM; SOFUOĞLU, KENAN; GHISOLFI, LAURA; and ÖKTEM, GÜLPERİ (2018) "Effects of di erent drug treatments on the proliferation of human ovarian carcinoma cell line MDAH-2774," Turkish Journal of Medical Sciences: Vol. 48: No. 2, Article 36. https://doi.org/10.3906/sag-1507-193
Available at: https://journals.tubitak.gov.tr/medical/vol48/iss2/36