Vitamin C as an antioxidant: evaluation of its role on pulmonary contusion experimental model

Authors

RANA SIRMALI
ZEYNEP GİNİŞ
MEHMET SIRMALI
OKAN SOLAK
BENGÜ ŞELİMAN
YETKİN AĞAÇKIRAN
NAMIK DELİBAŞ

DOI

10.3906/sag-1309-139

Abstract

To analyze the protective activity of vitamin C on the lungs by assessing biochemical and histopathological analysis after performing an experimental isolated lung contusion model. Materials and methods: Fifty-four male Sprague-Dawley male rats were used. The rats were randomly separated into 4 groups. Vitamin C (200 mg/kg) was injected intraperitoneally 30 min after trauma. Blood samples were obtained for myeloperoxidase (MPO), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities and malondialdehyde (MDA) levels. Blood gas analysis and bronchoalveolar lavage was performed. The lung tissue was also extracted for histopathological examination. Results: The lung contusion enhanced MDA, SOD, CAT, and MPO and diminished GSH-Px. Vitamin C administration after the pulmonary contusion was found to diminish the level of MDA and the activities of SOD, CAT, and MPO and to enhance the level of GSH-Px (P < 0.05). Contusion-induced disrupted gas analysis and leukocyte infiltration were both resolved by the vitamin C. Conclusion: The present results indicate that vitamin C administration attenuated the oxidative damage and morphological changes induced by pulmonary contusion in an experimental rat study.

Keywords

Vitamin C, antioxidant, lung injury, pulmonary contusion

First Page

905

Last Page

913

Recommended Citation

SIRMALI, RANA; GİNİŞ, ZEYNEP; SIRMALI, MEHMET; SOLAK, OKAN; ŞELİMAN, BENGÜ; AĞAÇKIRAN, YETKİN; and DELİBAŞ, NAMIK (2014) "Vitamin C as an antioxidant: evaluation of its role on pulmonary contusion experimental model," Turkish Journal of Medical Sciences: Vol. 44: No. 6, Article 1. https://doi.org/10.3906/sag-1309-139
Available at: https://journals.tubitak.gov.tr/medical/vol44/iss6/1