Anti-HDV enzyme immunoassay index in predicting HDV viremia in serum samples

Authors

SİBEL ÖZSU CAYMAZ
AYÇA ARZU SAYINER
AYŞE AYDAN ÖZKÜTÜK
YUSUF HAKAN ABACIOĞLU

DOI

10.3906/sag-1204-81

Abstract

To determine whether the total anti-delta antibody enzyme immunoassay (EIA) test results can be used to predict viremia. Materials and methods: The EIA index value of an anti-delta total antibody assay was determined in 76 serum samples obtained from 67 patients positive for the anti-hepatitis delta virus (HDV) antibody. The presence of HDV RNA was investigated by reverse transcriptase polymerase chain reaction (RT-PCR). The relation between the RT-PCR result and EIA index was evaluated by ROC analysis. Results: Of the 76 total samples, 56 (74%) anti-HDV-positive samples were found to be PCR-positive. The samples with EIA index values below 75.75 were PCR-negative, while samples with index values above 102.55 were PCR-positive. When HDV RNA presence is considered as the gold standard, the sensitivity, specificity, and positive and negative predictive values of the chosen EIA index (100.10) in predicting viremia were 93%, 80%, 93%, and 76%, respectively. Conclusion: HDV RNA is the most reliable marker for the diagnosis of active HDV infection, and EIA index value is closely related to viral RNA presence.

Keywords

Anti-HDV enzyme immunoassay, ROC analysis, HDV RNA, reverse transcriptase polymerase chain reaction

First Page

294

Last Page

298

Recommended Citation

CAYMAZ, SİBEL ÖZSU; SAYINER, AYÇA ARZU; ÖZKÜTÜK, AYŞE AYDAN; and ABACIOĞLU, YUSUF HAKAN (2013) "Anti-HDV enzyme immunoassay index in predicting HDV viremia in serum samples," Turkish Journal of Medical Sciences: Vol. 43: No. 2, Article 20. https://doi.org/10.3906/sag-1204-81
Available at: https://journals.tubitak.gov.tr/medical/vol43/iss2/20