Turkish Journal of Medical Sciences
DOI
-
Abstract
Some genetic diseases may have increased cellular instability. Since chronic myloid leukemia (CML) has a genetic base, we aimed to seek the genetic instability of patients by micronucleus analysis, a mutation screening test which was applied by cytokinesis-blocked (CB) method. In this study, 10 CML patients at diagnosis, were analysed for their micronuclei (MN) frequencies. The values were evaluated in different culture conditions of blood and bone marrow cells. The results of CML patients are compared with control' MN values obtained by peripheral blood cultures induced with PHA. Between bone marrow and peripheral blood cultures MN values with or without PHA among patients didn't show any significant difference. However, when these values compared with the control group all of them exhibited higher MN values in a statistically significant manner. It is suggested that this method is useful in the routine cytogenetic studies of leukemia.
Keywords
Micronucleus assay, cytochalasine B, bone marrow, CML.
First Page
125
Last Page
128
Recommended Citation
BAŞER, DİLEK KOÇ; SUNGUROĞLU, ASUMAN; BÖKESOY, IŞIK; and UYSAL, AKIN (1999) "Spontaneous micronuclei in cytokinesis-blocked bone marrow and peripheral blood Iymphocytes of CML patients," Turkish Journal of Medical Sciences: Vol. 29: No. 2, Article 9. Available at: https://journals.tubitak.gov.tr/medical/vol29/iss2/9
