Turkish Journal of Medical Sciences
DOI
-
Abstract
In this study, the in vitro effects of some antibiotics on carbonic anhydrase (CA) isozymes have been investigated. Human erythrocyte CA-I and CA-II isozymes were separately purified by affinity chromatography. Inhibition effects of four different antibiotics on CA isozymes were determined by using the CO 2 -Hydratase method. I 50 values of antibiotics were obtained by drawing % Activity [Actibiotic] graphs. The obtained I 50 values of cefazolin sodium, cephradine, sulbactam/cefoperazone and chloramphenicol sodium succinate were 9, 16, 19 and 48 mM on CA-I and 6, 10, 15 and 17 mM on CA-II, respectively. Antibiotics dosages should be taken into consideration because of the inhibition effect on CA which is pH regulatory enzyme in body.
Keywords
Carbonic anhydrase isozymes, antibiotics, inhibition.
First Page
407
Last Page
410
Recommended Citation
ÇOBAN, T. Abdülkadir; NALBANTOĞLU, Barbaros; ÇİL, M. Yaşar; ÖZDEMİR, Hasan; and KÜFREVİOĞLU, Ö. İrfan (1998) "Investigation of the Inhibition Effects of SomeAntibiotics on Human ErythrocyteCarbonic Anhydrase Isozymes," Turkish Journal of Medical Sciences: Vol. 28: No. 4, Article 13. Available at: https://journals.tubitak.gov.tr/medical/vol28/iss4/13
