Turkish Journal of Medical Sciences
An Enzymatic Method for Isolating Cardiac Myocytes from Heart of Guinea-pig Used for Single-Cell and Patch-Clamp Studies
Abstract
A method for isolating cardiac myocytes from the heart of guinea-pig is described. This method consists of perfusion of the heart with a low Ca{^2+} medium and subsequent dispersion of cells with collagenase and proteolytic enzyme. More than 80% rod-shoped, viable and quiescent cells which are suitable for electrophysiological and pharmacological experiments are obtained by this method.
DOI
-
Keywords
Isolated myocyte, guinea-pig, dissociation of cells.
First Page
565
Last Page
567
Recommended Citation
MERAL, İ (1997). An Enzymatic Method for Isolating Cardiac Myocytes from Heart of Guinea-pig Used for Single-Cell and Patch-Clamp Studies. Turkish Journal of Medical Sciences 27 (6): 565-567. https://doi.org/-
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