A New Method for the Purification of Carbonic Anhydrase Isozymes by Affinity Chromatography
A new affinity chromatography for purification of carbonic anhydrase isozymes was prepared by using Sepharose 4B as a matrix, L-tyrosine as a spacer-arm and p-aminobenzenesulfonamide as a ligand. The binding capacities of the affinity gel were determined at different temperatures, pH's and ionic strengths. As a result of the affinity gel having good flow properties and spacer arm, human carbonic anhydrase isozymes (HCA I and HCA ll) and bovine carbonic anhydrase (BCA) were purified in high yields from erythroyctes.
Carbonic anhydrase, isozymes, affinity chromatography
ARSLAN, 0ktay; NALBANTOĞLU, Barbaros; DEMİR, Nazan; ÖZDEMİR, Hasan; and KÜFREVİOĞLU, Ö. İrfan (1996) "A New Method for the Purification of Carbonic Anhydrase Isozymes by Affinity Chromatography," Turkish Journal of Medical Sciences: Vol. 26: No. 2, Article 10. Available at: https://journals.tubitak.gov.tr/medical/vol26/iss2/10