A New Method for the Purification of Carbonic Anhydrase Isozymes by Affinity Chromatography

Authors

0ktay ARSLAN
Barbaros NALBANTOĞLU
Nazan DEMİR
Hasan ÖZDEMİR
Ö. İrfan KÜFREVİOĞLU

DOI

-

Abstract

A new affinity chromatography for purification of carbonic anhydrase isozymes was prepared by using Sepharose 4B as a matrix, L-tyrosine as a spacer-arm and p-aminobenzenesulfonamide as a ligand. The binding capacities of the affinity gel were determined at different temperatures, pH's and ionic strengths. As a result of the affinity gel having good flow properties and spacer arm, human carbonic anhydrase isozymes (HCA I and HCA ll) and bovine carbonic anhydrase (BCA) were purified in high yields from erythroyctes.

Keywords

Carbonic anhydrase, isozymes, affinity chromatography

First Page

163

Last Page

166

Recommended Citation

ARSLAN, 0ktay; NALBANTOĞLU, Barbaros; DEMİR, Nazan; ÖZDEMİR, Hasan; and KÜFREVİOĞLU, Ö. İrfan (1996) "A New Method for the Purification of Carbonic Anhydrase Isozymes by Affinity Chromatography," Turkish Journal of Medical Sciences: Vol. 26: No. 2, Article 10. Available at: https://journals.tubitak.gov.tr/medical/vol26/iss2/10