In vitro anticancer, antioxidant and chelating activities of natural organosulfur compounds originated from Türkiye: an investigation on breast and colorectal cancer cells

Authors

ÜMMÜGÜLSÜM POLAT KORKUNÇFollow
HİLAL ÇALIKFollow
LEYLA POLAT KÖSEFollow
RABİA ÇAKIR KOÇFollow
EMİNE KARAKUŞFollow

Abstract

Background/aim:Taşköprü garlic, cultivated in the Taşköprü region of Kastamonu province, is a very famous in Türkiye.This study aimed to determine the anticancer and antioxidant effects of extracts from Kastamonu Taşköprü garlic on human breast cancer and colorectal cancer cells.Materials and methods:Taşköprü garlic, which contains natural organosulfur compounds, has a geographical registration. Taşköprü garlic, which contains natural organosulfur compounds (OSCs), has a geographical registration.Garlic contains oil- and water-soluble OSCs known to exhibit anticancer activity by interfering with MCF-7 and Caco-2 proliferation and tumor metastasis. In this study, the antiproliferative activity of oil- and water-soluble garlic extracts with and without glutathione at different concentrations was evaluated using the XTT assay on NIH/3T3, MCF-7, and Caco-2 cell lines for 24 hours. In addition, the reducing capacity,radical scavenging activity, and metal chelation activity of OSCs in Taşköprü garlic were analysed.Results:Both oil- and water-soluble garlic extracts significantly inhibited the proliferation of MCF-7 and Caco-2 in a dose-dependent manner after a 24 h incubation.The half-maximal inhibition concentration (IC50) values of OSCs and positive controls for N,N- dimethyl-p-phenylenediamine cation (DMPD•+) radical removal and 2,2'-bipyridyl-Fe2+ chelation activity were 129.593-1004.346 and 165.065-495.195 μg/mL, respectively.Then, the reducing impact of OSCs and positive controls were evaluated by the cupric ion (Cu2+) reducing capabilities.All reported results were compared with the positive controls.Conclusion:The findings revealed that oil-soluble garlic extracts exhibited anticancer properties against both Caco-2 and MCF-7 cancer cells, without inducing any cytotoxicity in non-cancerous NIH/3T3.In addition, water-soluble extracts have higher antiproliferative activity on Caco-2 and MCF-7 cells in a dose-dependent manner compared to oil-soluble extracts, nevertheless, they also showed notable cytotoxicity on fibroblast cells.OSCs were not very active in reduction and radical scavenging tests,but their chelating activity was significantly good.These results suggested that both water-soluble and oil-soluble garlic extracts have a promising anticancer potential against both MCF-7 and Caco-2.

Author ORCID Identifier

ÜMMÜGÜLSÜM POLAT KORKUNÇ: 0000-0001-6942-9532

HİLAL ÇALIK: 0000-0002-9509-7866

LEYLA POLAT KÖSE: 0000-0001-5759-7889

RABİA ÇAKIR KOÇ: 0000-0002-8545-9878

EMİNE KARAKUŞ: 0000-0002-7730-3304

DOI

10.55730/1300-0144.5970

Keywords

antioxidant activity, breast cancer, cell viability, colorectal cancer, Kastamonu Taşköprü garlic, metal chelating capacity, organosulfur compounds

First Page

287

Last Page

298

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

POLAT KORKUNÇ, ÜMMÜGÜLSÜM; ÇALIK, HİLAL; POLAT KÖSE, LEYLA; ÇAKIR KOÇ, RABİA; and KARAKUŞ, EMİNE (2025) "In vitro anticancer, antioxidant and chelating activities of natural organosulfur compounds originated from Türkiye: an investigation on breast and colorectal cancer cells," Turkish Journal of Medical Sciences: Vol. 55: No. 1, Article 34. https://doi.org/10.55730/1300-0144.5970
Available at: https://journals.tubitak.gov.tr/medical/vol55/iss1/34