Turkish Journal of Medical Sciences
Evaluation of syringin?s neuroprotective effect in a model of neonatal hypoxic-ischemic brain injury
DOI
10.55730/1300-0144.5697
Abstract
Background/aim: A significant cause of mortality and morbidity in the neonatal era is hypoxic-ischemic encephalopathy (HIE). This study examined the histopathological analysis and neuroprotective impact of syringin (SYR) in an experimental HIE rat model. Material and methods: On the 7th postnatal day, 24 Wistar albino rats were evaluated in 3 groups using the HIE model under gas anesthesia. In the experiment, Group A received 10 mg/kg SYR plus dimethyl sulfoxide (DMSO), Group B received DMSO only, and Group C served as a sham group. Immunohistochemical techniques were used to assess apoptotic cell measurement and proinflammatory cytokines (TNF-? and IL-1ß primary antibodies). Results: Rats suffering from hypoxic-ischemic brain damage had their apoptosis assessed. The SYR and sham groups had statistically fewer cells undergoing apoptosis (p < 0.001). There was no difference between the groups in terms of IL-1ß and TNF-? during immunohistochemical staining. Neuronal degeneration was significantly lower in the histological evaluation of the hippocampus in the SYR group (p = 0.01). A statistically significant difference (p = 0.01) was observed between the SYR and the control groups regarding pericellular and perivascular edema. Conclusion: SYR reduced apoptosis, perivascular and pericellular edema, and neuronal degeneration in rat cerebral tissue. These results raise the possibility that SYR may have a neuroprotective effect on the harm brought on by HIE. This is the first investigation of SYR?s function within the HIE paradigm.
Keywords
Hypoxic-ischemic, brain injury, antiinflammatory, apoptosis, syringin
First Page
1312
Last Page
1320
Recommended Citation
ERGON, EZGİ YANGIN; ÇELİK, ASLI; ÜNLÜ, AYŞE GÜLDEN DİNİZ; ÇOLAK, RÜYA; ÖZDEMİR, SENEM ALKAN; ÇALKAVUR, ŞEBNEM; and YILMAZ, OSMAN
(2023)
"Evaluation of syringin?s neuroprotective effect in a model of neonatal hypoxic-ischemic brain injury,"
Turkish Journal of Medical Sciences: Vol. 53:
No.
5, Article 32.
https://doi.org/10.55730/1300-0144.5697
Available at:
https://journals.tubitak.gov.tr/medical/vol53/iss5/32