Turkish Journal of Medical Sciences
Abstract
Background/aim: The effect of testosterone replacement therapy was investigated on bladder functions, histology, apoptosis as well as Rho-kinase expression in the rat bladder outlet obstruction (BOO) and hypogonadism models. Materials and methods: 30 mature male rats divided into 4 groups: sham group (n = 8), BOO group (n = 8), BOO + orchiectomy group (n = 7), BOO + orchiectomy + testosterone (T) treatment group (n = 7). Cystometric findings, apoptosis index, Rho-kinase (ROCK-2) expression, and smooth muscle/collagen ratio were compared. Results: BOO did not change ROCK-2 expression level, compared to sham group (P > 0.05). However, when compared to BOO group (P < 0.01), BOO + orchiectomy led ROCK-2 increase. The testosterone treatment failed to reverse the up-regulation of ROCK-2 induced by orchiectomy although it tended to lower ROCK-2 level. Compared to sham group (P = 0.002), changes in maximal bladder capacity and leak point pressure were higher (P = 0.026, P = 0.001), and bladder compliance was lower in BOO group. Also, the apoptosis index was different between the two groups (P = 0.380). Smooth muscle/collagen ratio was higher in BOO + orchiectomy + T group than in BOO + orchiectomy group (P = 0.010). Conclusions: The research draws attention to alternating treatment approaches in case of the presence of hypogonadism and BOO.
DOI
10.3906/sag-2004-15
Keywords
Bladder, rho-kinase expression, hypogonadism, apoptosis, bladder outlet obstruction
First Page
1491
Last Page
1499
Recommended Citation
SAYLAM, B, EFESOY, O, ARPACI, R. B, TİFTİK, R. N, TEK, M, BÜYÜKAFŞAR, K, BOZLU, M, & ÇAYAN, S (2021). The effect of testosterone replacement therapy on bladder functions, histology, apoptosis,and Rho-kinase expression in bladder outlet obstruction and hypogonadism rat model. Turkish Journal of Medical Sciences 51 (3): 1491-1499. https://doi.org/10.3906/sag-2004-15