Turkish Journal of Medical Sciences
Is cervical swab an efficient method for developing a new noninvasive prenatal diagnostictest for numerical and structural chromosome anomalies?
Background/aim: Prenatal diagnosis is vital to obtain healthy generation for risky pregnancies. There have been several approaches, some of which are routinely applied in clinics to evaluate the possible prenatal deficiencies and/or diseases. In the present study, we aimed to isolate the fetal cells from endocervical samples and try to identify possible anomalies which were proved by Amniocentesis (AS) and chorionic villus sampling (CVS) methods. Materials and methods: Endoservical specimens were collected from 100 pregnant women. Cells were separated in parallel by fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS) using human leukocyte antigen (HLA) G233 and placental alkaline phosphatase (PLAP) antibodies. CMA (comprehensive meta-analysis) were carried out and male fetuses were confirmed with Sex determining region Y (SRY) amplification. Results: The percent of HLA G233 and placental and placental alkaline phosphatase (PLAP) positive cells were 4.55% and 84.59%, respectively. The percent of cells positive for both markers was 14.75%. CMA analyses were not informative. (SRY) was amplified in 67% of the samples. Conclusion: However, the success rate of the both cell sorting and scanning of DNA anomalies by aCGH and/or RT-PCR was limited, preventing the applicability of this proposal in the clinics. Still, the success of the proposed method depends on the development of the novel fetal cell-specific antibodies and the improvements in the sorting systems.
Cervical swab, CMA (comprehensive meta-analysis), fluorescence-activated cell sorting (FACS), magnetic-activated cell sorting (MACS)
YURTCU, ERKAN; KARÇAALTINCABA, DENİZ; KAZAN, HASAN HÜSEYİN; ÖZDEMİR, HALİS; KARAOĞUZ, MERAL YİRMİBEŞ; ÇALIŞ, PINAR TOKDEMİR; KAYHAN, GÜLSÜM; ERGÜN, SEZEN GÜNTEKİN; PERÇİN, FERDA EMRİYE; BAYRAM, MERİH; İLHAN, MUSTAFA NECMİ; DEDEOĞLU, GAMZE BİLGİLİ; KAYMAK, TUĞRUL; and ERGÜN, MEHMET ALİ
"Is cervical swab an efficient method for developing a new noninvasive prenatal diagnostictest for numerical and structural chromosome anomalies?,"
Turkish Journal of Medical Sciences: Vol. 51:
3, Article 18.
Available at: https://journals.tubitak.gov.tr/medical/vol51/iss3/18