Turkish Journal of Medical Sciences
Abstract
Background/aim: To evaluate the effects of hysterectomy on rat ovaries and the possible protective role of peroxisome proliferator-activated receptor gamma (PPAR-?) agonist-rosiglitazone against ovarian reserve decrement.Materials and methods: Forty-five adult Wistar albino rats were randomly divided into three groups. Hysterectomy was performed (n = 15) in group 1 [H]; 1 mg/kg/day PPAR-? agonist/rosiglitazone was used for 50 days after hysterectomy (n = 15) in group 2 [H + R]; a sham operation was performed (n = 15) in group 3 [control, C]. Blood samples were collected for anti-Müllerian hormone (AMH) evaluation in all groups and simultaneous ovarian Doppler examination was performed in [H] and [H + R] groups before and after (50 days) hysterectomy. All animals were sacrificed to obtain ovaries for histological examination.Results: AMH levels were found to be significantly decreased at postoperative day 50 in all groups (P < 0.05). Histopathologic analysis showed that primary, preantral, and antral follicle counts were significantly higher in the [H] group as compared to the [C] and [H + R] groups (P < 0.05). There was no significant difference between the [C] and [H + R] groups in terms of follicle numbers (P > 0.05). In the ovarian Doppler blood flow analysis, all parameters were significantly decreased in group [H] (P < 0.05), but not in the [H + R] group (P > 0.05) on postoperative day 50. Conclusion: Hysterectomy affects the histopathological structure of rat ovaries and PPAR-? agonist-rosiglitazone improves the ovarian Doppler blood flow parameters.
DOI
10.3906/sag-2002-117
Keywords
Hysterectomy, ovarian reserve, rat, Doppler ultrasonography, PPAR gamma, rosiglitazone
First Page
1399
Last Page
1408
Recommended Citation
ÖZTÜRK, B. G, TAPISIZ, Ö. L, DİLBAZ, B, MÜLAZIMOĞLU, S. B, MÜFTÜOĞLU, K. H, DÜNDAR, B, & GÖKTOLGA, Ü (2020). What is the impact of PPAR-? agonist-rosiglitazone on ovarian reserve after hysterectomy? An experimental study. Turkish Journal of Medical Sciences 50 (5): 1399-1408. https://doi.org/10.3906/sag-2002-117