Turkish Journal of Medical Sciences
Abstract
Background/aim: The present study was designed to explore antidiabetic and hepatoprotective potentials of Fraxinus angustifolia leaf (FAL) and bark (FAB) extracts in vivo. Materials and methods: Streptozotocin (STZ)-induced diabetic rats, pretreated with the extracts (25 and 50 mg/kg), were monitored for fasting blood glucose (FBG) levels. Hepatoprotective potential was examined after injection of an excessive dose of paracetamol (10 g/60 kg) by analysis of biochemical parameters (transaminases, bilirubin), malondialdehyde (MDA) levels, and histological sections. high performance liquid chromatography analysis was also performed for partial characterization. Results: A considerable hypoglycemic effect was noticed 2 h after the STZ-induction, with a higher efficiency (P < 0.05) for FAL (68%) as compared with FAB (57%). A significant (P < 0.05) reduction in MDA was observed for paracetamol-fed mice pretreated with FAL (50 mg/kg), FAB (50 mg/kg), or both (25 mg/kg each) extracts, and the MDA levels for the three conditions were 0.290 ± 0.034, 0.340 ± 0.038, and 0.25 ± 0.058 nmoles/mg of liver tissue, respectively). Hence, simultaneous treatment provided a better protection. Histological observations confirmed the higher hepatoprotective potential of FAL over FAB extracts Conclusion: The obtained results indicate the possibility of pharmacological exploitation of F. angustifolia extracts in the treatment of diabetes and associated liver diseases.
DOI
10.3906/sag-1502-13
Keywords
Fraxinus angustifolia, antidiabetic, hepatoprotective, lipid peroxidation, ?-amylase
First Page
910
Last Page
920
Recommended Citation
MEDJAHED, Z, ATMAN-KILANI, D, FAUCONNIER, M, RICHARD, G, & ATMANI, D (2016). Hepatoprotective and antidiabetic activities of Fraxinus angustifolia Vahl extracts in animal models: characterization by high performance liquid chromatography analysis. Turkish Journal of Medical Sciences 46 (3): 910-920. https://doi.org/10.3906/sag-1502-13