Turkish Journal of Medical Sciences
In vitro efficacy of frozen erythrocytes: implementation of new strategicblood stores to alleviate resource shortage (issue revisited)
Background/aim: Currently, the provision of blood products largely depends on walking blood banks and limited amounts of stored blood with short shelf lives. We aimed to compare the efficacy of erythrocyte concentrate (ECs) by pre- and postfreezing in vitro tests. Materials and methods: In our study, 10 ECs were glycerolized, frozen, thawed, and then deglycerolized using the Naval Blood Research Laboratory method. In addition to using the standard tests, ATP and 2,3-DPG levels and the viability of erythrocytes were also determined. Results: The prefreezing mean viability rates of erythrocytes changed from 89.7 ± 13.7% to 98.6 ± 1.8% after thawing and deglycerolization. Prefreezing and day 0 ATP levels (1.64 ± 0.15 µmol/g Hb and 1.81 ± 0.14 µmol/g Hb, respectively) were similar. The 2,3-DPG levels decreased from 18.09 ± 4.78 µmol/g Hb measured before the procedure to 10.41 ± 4.58 µmol/g Hb on day 0. The mean hemolysis rates and supernatant Hb levels changed from 0.21 ± 0.11% to 0.36 ± 0.12% and 1 ± 0.5 g/L to 1.5 ± 0.5 g/L, respectively. Conclusion: The test results showed the efficacy of the frozen-thawed ECs to be used in humans for a broad spectrum of clinical indications. As a part of a contingency plan, national frozen blood reserves need to be established.
Cryopreservation, erythrocyte concentrates, blood transfusion, flow cytometric analysis
ÇETİNKAYA, RIZA AYTAÇ; YILMAZ, SONER; EKER, İBRAHİM; ÜNLÜ, AYTEKİN; UYANIK, METİN; TAPAN, SERKAN; PEKOĞLU, AHMET; PEKEL, ALİYE AYSEL; ERTAŞ, ZERRİN; GÜRSEL, ORHAN; MUŞABAK, UĞUR HACİ; YILMAZ, SEBAHATTİN; AVCI, İSMAİL YAŞAR; ÇETİN, AHMET TÜRKER; and EYİGÜN, CAN POLAT
"In vitro efficacy of frozen erythrocytes: implementation of new strategicblood stores to alleviate resource shortage (issue revisited),"
Turkish Journal of Medical Sciences: Vol. 45:
3, Article 25.
Available at: https://journals.tubitak.gov.tr/medical/vol45/iss3/25