Turkish Journal of Medical Sciences




To study the prevalence of bla_{VIM} and bla_{IMP} genes in metallo-\beta-lactamase (MBL)-producing burn isolates of Pseudomonas aeruginosa in relation with AmpC and extended-spectrum \beta-lactamase (ESBL) production. Materials and methods: Thirty-two carbapenem-resistant MBL-producing P. aeruginosa burn isolates from Shahid Motahari Burn Hospital in Tehran were employed. Antibiotic susceptibility was determined to 13 antibiotics including imipenem and meropenem by disk diffusion. AmpC and ESBL production was detected by the AmpC disk test and combined disk diffusion assay, respectively. bla_{IMP} and bla_{VIM} gene carriage was shown by polymerase chain reaction and type-specific primers. Results: AmpC production was observed in 81% and ESBL production was detected in 12.5% of the isolates. bla_{IMP} carriage was observed in 56.25% and bla_{VIM} gene in 46.8% of the isolates. Surprisingly, 43.5% of the isolates carried both bla_{IMP} and bla_{VIM} genes. Conclusion: We think that this is the first report on the cocarriage of bla_{IMP} and bla_{VIM} in P. aeruginosa. There was also a strong association between MBL gene carriage and AmpC \beta-lactamase production.


Pseudomonas aeruginosa, metallo-\beta-lactamase, MBL, bla_{IMP}, bla_{VIM}, AmpC, ESBL

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