To detect the prevalence and type distribution of human papillomavirus (HPV) in different cytological diagnostic categories. Materials and methods: Between 2007 and 2010, a total of 1014 liquid-based thin preparations of cervical smears were selected and classified according to cytology results. HPV DNA polymerase chain reaction (PCR) was also performed using these samples. HPV DNA-positive samples were genotyped by DNA sequencing. Results: Of those enrolled in the study, 45.3% were negative cytologically, 36.4% had atypical squamous cells of undetermined significance, 0.3% had atypical squamous cells preventing the exclusion of a high-grade squamous intraepithelial lesion, 16.8% showed a low-grade squamous intraepithelial lesion, and 1.3% had an high-grade squamous intraepithelial lesion. Conclusion: PCR assays showed HPV positivity in 63.0% of cytologically negative and 90.8% of cytologically positive samples. The most common types of HPV detected were 16, 6, 18, 31, 66, 56, 53, 81, 45, and 62. Of HPV DNA-positive samples, 47.7% were high, 4.7% were intermediate, and 17.9% were low risk. The high-risk types of HPV detected were 16, 18, 31, 56, 53, 45, 62, 58, 59, 67, 51, 35, 73, 52, 33, 39, 68, and 82.
Cytology, human papillomavirus DNA, genotype, polymerase chain reaction
AKYAR, IŞIN; AYDIN, ÖZLEM; YAKICIER, MUSTAFA CENGİZ; KOCAGÖZ, ZÜHTÜ TANIL; İNCE, ÜMİT; and ÜNSAL, İBRAHİM
"Human papillomavirus prevalence and type in liquid-based cervical samples from Turkish women in a selected risk group,"
Turkish Journal of Medical Sciences: Vol. 43:
6, Article 15.
Available at: https://journals.tubitak.gov.tr/medical/vol43/iss6/15