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Turkish Journal of Medical Sciences

DOI

10.3906/sag-1009-1168

Abstract

To investigate the association between the paraoxonase 1 (PON1) c.163T>A and c.575A>G polymorphisms and coronary artery disease (CAD), and whether specific polymorphisms in the PON1 gene are associated with CAD in the southeastern Turkish population. Many different genetic and clinical factors have been identified as causes or contributors to atherosclerosis. Complex diseases such as CAD, hypertension, and diabetes are usually caused by an individual’s susceptibility to various genes, environmental factors, and the interactions between them. The PON1 enzyme has been implicated in the pathogenesis of atherosclerosis and CAD. Materials and methods: Enrolled in this study were 61 patients with CAD and 103 healthy individuals; their DNA was isolated. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to determine the frequencies of the PON1 gene (NM_000446.5, GI: 5444) c.163T>A (Hsp92IIT>A) and c.575A>G (AlwIA>G) polymorphisms in CAD cases. Results: Our data indicated that the body mass index (BMI) (27.5 ± 5.7), low-density lipoprotein (LDL-C) values (127.0 ± 45.8), and frequencies of diabetes (13.0) and hypertension (35.0) were significantly higher in CAD patients than in the control subjects (25.9 ± 4.1; 105.6 ± 26.3; 2.0; and 19.0, respectively) (P < 0.05). No significant differences were observed in the frequencies of the c.163T>A and c.575A>G genotypes of the PON1 gene in CAD patients compared to healthy individuals (P > 0.05). Conclusion: The PON1 gene c.163T>A and c.575A>G polymorphisms did not represent an important risk factor for this disease in the southeastern Turkish population.

Keywords

PON1 gene, polymorphism, coronary artery disease, PCR, RFLP

First Page

895

Last Page

902

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