Ethidium Bromide Spot Test Is a Simple Yet Highly Accurate Method in Determining DNA Concentration

Authors

MURAT KASAP
SİNEM TOROL
GÜLÇİN GACAR
FATMA BUDAK

DOI

-

Abstract

A commonly used method to measure the amount of DNA in nucleic acid preparations is to use UV irradiation. However, if the DNA sample contains impurities, measuring UV irradiation is misleading. A quick way to estimate the amount of DNA in such samples is to analyze spot densities created by UV-induced fluorescence emitted by ethidium bromide. The amount of DNA is proportional to the amount of fluorescence. In many references, the spot density analysis is recommended for rough estimation of DNA concentration and is done by visual examination and comparison of the densities of each spot with the densities of DNA standards. Herein, we used a computer-integrated software to test the reliability of such a method, and showed that the analysis is highly reliable and simple.

Keywords

DNA concentration, spot density analysis, ethidium bromide

First Page

383

Last Page

386

Recommended Citation

KASAP, MURAT; TOROL, SİNEM; GACAR, GÜLÇİN; and BUDAK, FATMA (2006) "Ethidium Bromide Spot Test Is a Simple Yet Highly Accurate Method in Determining DNA Concentration," Turkish Journal of Medical Sciences: Vol. 36: No. 6, Article 11. Available at: https://journals.tubitak.gov.tr/medical/vol36/iss6/11