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Turkish Journal of Medical Sciences

DOI

-

Abstract

Aim: The purpose of this experimental study was to investigate the effect of pre-injury supplementation with vitamin E or selenium on antioxidant enzyme and malondialdehyde levels, as markers of lipid peroxidation, after thermal injury to rats. Materials and Methods: The animals were divided into 3 groups: Group 1 (n = 15): controls, no supplementation; Group 2 (n = 14): received vitamin E (100 mg/kg per day) for 10 days pre-burn; Group 3 (n = 14): received selenium (4 ppm in drinking water) for 10 days pre-burn. All animals were given second-degree burns. Serum and liver tissues of the rats were sampled 3 d after being burned. Serum and liver tissue superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione S-transferase (GST) activity and malondialdehyde (MDA) levels were measured. Results: Group 2 had significantly higher serum and liver tissue SOD, GSH-Px, and liver tissue CAT activity when compared to Group 1. In contrast, serum and tissue MDA levels and tissue GST activity were significantly lower in Group 1. Group 3 had greater serum and liver tissue SOD and GSH-Px activity, and lower MDA levels compared to Group 1. Conclusions: These data revealed that supplementation with vitamin E or selenium during the pre-injury period decreased lipid peroxidation and increased liver tissue SOD, GSH-Px, CAT, serum SOD, and GSH-Px activity following thermal injury to rats. Thus, supplementation with vitamin E or selenium, at appropriate doses, for patients at high risk of oxidative damage, such as surgical patients, may have beneficial effects.

Keywords

Selenium, vitamin E, antioxidant enzyme, malondialdehyde, burn injury

First Page

141

Last Page

146

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