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Turkish Journal of Medical Sciences

DOI

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Abstract

The purpose of this study was to determine the influence of various types of dental casting alloys and ceramic upon cell viability and the synthesis of IL-1beta (\beta) in a three-dimensional cell culture system consisting of human gingival fibroblast, and to determine their effect in gingival inflammation. Au-Pt-In alloy (Pontostar), Ni-Cr-Mo alloy (Remanium-CS), a titanium alloy (Ti-6Al-4V), copper (Cu), and an all ceramic (In-Ceram) were used as test materials. The materials were exposed to a three dimensional cell culture in order to determine their effect on cell viability and IL-1 (\beta) secretion level. Cell viability was measured by MTT test after exposure for 24h and 48h. For IL-1 (\beta) measurement, assay aliquots were taken from exposed media after 1h, 3h, 7h, 24h and 48h. Assays for IL-1 (\beta) were carried out by ELISA. In-Ceram, Pontostar and Ti-6Al-4V alloy did not influence cell viability. Copper (52-64%), and Remanium CS (17-20%) were found to be cytotoxic, compared to control cultures. In-Ceram, Pontostar and Ti-6Al-4V had no significant influence on IL-1 (\beta) secretion. Remanium-CS increased IL-1(\beta) levels two fold compared to untreated control cultures. Our findings suggested that Ti-6Al-4V, In-Ceram and Pontostar did not cause elevated IL-1 (\beta) release from cells at non-toxic levels. On the other hand, Remanium-CS showed moderate toxicity and caused increase in IL-1 (\beta) levels.

Keywords

Cell viability, MTT, IL-1 (\beta), Dental alloys, Three-dimensional cell culture

First Page

203

Last Page

208

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