Turkish Journal of Medical Sciences




In this study, the in vitro effects of some antibiotics on carbonic anhydrase (CA) isozymes have been investigated. Human erythrocyte CA-I and CA-II isozymes were separately purified by affinity chromatography. Inhibition effects of four different antibiotics on CA isozymes were determined by using the CO 2 -Hydratase method. I 50 values of antibiotics were obtained by drawing % Activity [Actibiotic] graphs. The obtained I 50 values of cefazolin sodium, cephradine, sulbactam/cefoperazone and chloramphenicol sodium succinate were 9, 16, 19 and 48 mM on CA-I and 6, 10, 15 and 17 mM on CA-II, respectively. Antibiotics dosages should be taken into consideration because of the inhibition effect on CA which is pH regulatory enzyme in body.

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