Turkish Journal of Medical Sciences
DOI
-
Abstract
The effects of anti-hipertensive phenoxybenzamine were in-vestigated on tyrosine hydroxylase (TH) enzyme activity and TH mRNA levels. In the present study 5 months male sprague dawley (SD) rats were used. Phenoxybenzamine was injected i.p as 20 mg/kg which prepared in the 0.9 % NaCl and 5% ethanole. TH activity was measured by detecting of formation of 3 H 2 O as a formation of dopa from 3 H-tyrosine by radioisotope technique total adrenal RNA was isolated and hybridized wit 32 P labeled cDNA. TH mRNA was assayed by densitometric scanning of the autoradiograms using a densitometer. TH activity and TH mRNA levels were found to be significantly increased by the effcet of phenoxybenzamine (P
Keywords
Phenoxybenzamine, tyrosine hydroxylase, adrenal medulla, TH mRNA
First Page
35
Last Page
40
Recommended Citation
YÜREKLİ, Muhittin (1998) "The Effects of Phenoxybenzamine on TyrosineHydroxylase (TH) and TH mRNA Level in AdrenalMedulla of Sprague Dawley Rats," Turkish Journal of Medical Sciences: Vol. 28: No. 1, Article 7. Available at: https://journals.tubitak.gov.tr/medical/vol28/iss1/7
