Turkish Journal of Medical Sciences
Antioxidant Role of Bovine Albumin Detected by a Chemiluminescence Method
DOI
-
Abstract
The antioxidant potency of albumin was tested in an experimental system by using a chemiluminescence method. The incubation medium consisted of hemoglobin, luminol, cumene hydroperoxide (CumOOH) or t-butyl hydroperoxide (t-BuOOH) and different concentrations of albumin in phosphate-buffered saline (PBS, pH 7.4). Different concentrations of albumin in the incubation medium were exposed to the same concentrations of CumOOH or t-BuOOH. The formation of chemiluminescence was detected in single-photon mode using a liquid scintillation counter. 5 mg/dL and higher concentrations of albumin that were exposed to t-BuOOH and 2 mg/dL ad higher concentrations of albumin that were exposed to Cum- OOH prevented chemiluminescense production. These observations support the antioxidant function of albumin.
Keywords
Chemiluminescence, Albumin, Cumene Hydroperoxide, t-Butyl Hydroperoxide.
First Page
431
Last Page
434
Recommended Citation
GÜMÜŞLÜ, Saadet; DEMİR, Ayşe Yasemin; YÜCEL, Gültekin; YEŞİLKAYA, Akın; AYDIN, Mutay; and AKSU, Tevfik Aslan (1996) "Antioxidant Role of Bovine Albumin Detected by a Chemiluminescence Method," Turkish Journal of Medical Sciences: Vol. 26: No. 5, Article 3. Available at: https://journals.tubitak.gov.tr/medical/vol26/iss5/3
