Detection of Serum HCV RNA by Polymerase Chain Reaction in Patients with Chronic Hepatitis C
Hepatitis C virus (HCV) RNA was isolated from the sera of 15 anti-HCV positive patients. cDNAs were synthesized by reverse transcription and amplified by two round nested polymerase chain reaction (PCR). HCV infection was confirmed by the detection of 256 base pairs long DNA fragments on the 2% agarose gels in the electrophoresis of PCR products with the positive and negative controls. 10 of 15 infection were confirmed by this molecular technique.
ÖZER, S. Ayşe; KALAYCI, Cem; AVŞAR, Melike; CANER, Müge; ÜLGEN, Sertaç Kip; AVŞAR, Erol; KIZILTAŞ, Şafak; and ÇIRAKOĞLU, Beyazıt (1996) "Detection of Serum HCV RNA by Polymerase Chain Reaction in Patients with Chronic Hepatitis C," Turkish Journal of Medical Sciences: Vol. 26: No. 3, Article 14. Available at: https://journals.tubitak.gov.tr/medical/vol26/iss3/14