Mutation Detection Using Unlabelled Amplification and Mismatch Detection (AMD) Analysis Technique

Authors

Munis DÜNDAR
Hasan ACAR
Hüseyin YÜCE
W. George LANYON
J. Michael CONNOR

DOI

-

Abstract

In this study unlabelled amplification mismatch analysis (AMD) method was used to detect mutations in the retinoblastoma gene (RB1). In order to compare the efficiency of the unlabelled method, unlabelled AMD analysis of RT-PCR amplified products was performed on patients with this bilateral retinoblastoma. This method was found to be fast and reliable and can be used for diagnostic purposes in countries where radiolabelled subtances are not readily available.

Keywords

Silver staining, AMD, RB1, mutation, RNA, cDNA

First Page

281

Last Page

284

Recommended Citation

DÜNDAR, Munis; ACAR, Hasan; YÜCE, Hüseyin; LANYON, W. George; and CONNOR, J. Michael (1996) "Mutation Detection Using Unlabelled Amplification and Mismatch Detection (AMD) Analysis Technique," Turkish Journal of Medical Sciences: Vol. 26: No. 3, Article 13. Available at: https://journals.tubitak.gov.tr/medical/vol26/iss3/13