The Chemiluminescence Detection of Protective Effect of Extracellularly Added NADPH and NADH on Erythrocytes Against Cumene Hydroperoxide-Induced Oxidative Stress

Authors

Gültekin YÜCEL
Saadet GÜMÜŞLÜ
Akın YEŞİLKAYA
Hasan AKIN
Aslan AKSU

DOI

-

Abstract

The purpose of this study was examination of the effect of the reduced nicotinamid adenine dinucleotide phosphate (NADPH) and reduced nicotinamid adenine dinucleotide (NADH) on erythrocytes against cumene hydroperoxide induced oxidative stress. Erythrocytes of 18 healthy volunteers were incubated with cumene hydroperoxide in the presence and absence of NADPH or NADH. Final concentrations in the incubation medium were hemoglobin 0.05 g/dl, luminol 3.75 \mumol/L, cumene hydroperoxide 125 \mumol/L, NADPH or NADH 0.1 mmol/L. Extracellularly added NADPH and NADH extensively diminished luminol amplified chemiluminescence induced by cumene hydroperoxide and retarded maximum peroxidation time (P

Keywords

NADH, NADPH, erythrocytes, oxidative stress, cumene hydroperoxide.

First Page

187

Last Page

191

Recommended Citation

YÜCEL, Gültekin; GÜMÜŞLÜ, Saadet; YEŞİLKAYA, Akın; AKIN, Hasan; and AKSU, Aslan (1996) "The Chemiluminescence Detection of Protective Effect of Extracellularly Added NADPH and NADH on Erythrocytes Against Cumene Hydroperoxide-Induced Oxidative Stress," Turkish Journal of Medical Sciences: Vol. 26: No. 2, Article 15. Available at: https://journals.tubitak.gov.tr/medical/vol26/iss2/15